转appA-MxA基因猪PCR检测灵敏度及环境安全评价研究

doi:10.11843/j.issn.0366-6964.2014.09.003

Abstract

Abstract:

This experiment was conducted to analyze PCR detection ability of exogenous gene in appA-MxA transgenic pigs and to study the possibility of exogenous gene drifting to the environment through feces.To analyze PCR detection ability of exogenous gene，a series of pcDNA-appA-MxA plasmids 10-fold diluted were amplified by PCR which taking primers designed according to appA and MxA genes in appA-MxA co-transgenic pig.Appropriate fecal DNA in transgenic pigs also be amplified by PCR to detect gene drift between feces and environment.Results of PCR showed that，the minimal concentration of CMV-appA and CMV-MxA in 10 μL PCR system were 2.4×10⁴ and 2.4×10³ copy•μL^-1，respectively.Detection results of appropriate fecal DNA from transgenic pigs which could overcome PCR inhibition showed that appA and MxA were not existed in feces from appA-MxA co-transgenic pigs.These results indicated that exogenous genes in transgenic pig could hardly be drifted into the environment through feces，and to some extent，transgenic pigs are safe to the environment.

CLC Number:

S828
S813.3

LIU Xiao-dan，XIN Lei-lei，FAN Jun-hua，KONG Si-yuan，YANG Shu-lin，LI Kui. Analysis on PCR Detection Ability and Environmental Safety Assessment in appA-MxA Co-transgenic Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1399-1403.

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Analysis on PCR Detection Ability and Environmental Safety Assessment in appA-MxA Co-transgenic Pigs

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